Antiviral activity of Mx proteins from gilthead seabream (Sparus aurata) against lymphocystis disease virus (LCDV)

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Gilthead seabream - Sparus aurata

Distribution and capture Th e gilthead seabream, Sparus aurata, is a subtropical Sparidae distributed from 62°N 15°N, 17°W 43°E. It occurs naturally in the Mediterranean and the Black Sea (rare), and in the Eastern Atlantic, from the British Isles, Strait of Gibraltar to Cape Verde and around the Canary Islands (1). Gilthead sea bream are captured with traditional and sporting equipment, and so...

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Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata)

The lymphocystis disease (LCD), the main viral pathology described in cultured gilthead seabream (Sparus aurata), is a self-limiting condition characterized by the appearance of hypertrophied fibroblasts (named lymphocysts) in the connective tissue of fish, primarily in the skin and fins. The causative agent of the disease is the Lymphocystis disease virus (LCDV), a member of the Iridoviridae f...

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Detection of lymphocystis disease virus (LCDV) in asymptomatic cultured gilt-head seabream (Sparus aurata, L.) using an immunoblot technique.

An immunoblot technique for the detection of lymphocystis disease virus (LCDV) in naturally infected gilt-head seabream (Sparus aurata, L.) has been developed. A specific antiserum against a 60 kDa viral protein has been proven to be an appropriate tool for LCDV diagnosis either from inoculated cell cultures or from fish tissues using the immunoblot assay. The sensitivity of this technique vari...

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Application of a new real-time polymerase chain reaction assay for surveillance studies of lymphocystis disease virus in farmed gilthead seabream.

BACKGROUND Lymphocystis disease (LCD) is the main viral infection reported to affect cultured gilthead seabream (Sparus aurata) in Europe. The existence of subclinical Lymphocystis disease virus (LCDV) infection in this fish species has been recognised by using polymerase chain reaction (PCR)-based methods. Nevertheless, these methods do not provide quantitative results that can be useful in ep...

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Protein and glycoprotein content of lymphocystis disease virus (LCDV).

The polypeptide and glycoprotein composition of eight strains of the fish-pathogenic lymphocystis disease virus (LCDV) isolated from gilt-head seabream (Sparus aurata), blackspot seabream (Pagellus bogaraveo), and sole (Solea senegalensis) were determined. The protein electrophoretic patterns of all LCDV isolates were quite similar regardless of the host fish, showing two major proteins (79.9 a...

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ژورنال

عنوان ژورنال: Fish & Shellfish Immunology

سال: 2013

ISSN: 1050-4648

DOI: 10.1016/j.fsi.2013.03.052